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September 28, 2006

Testing of Condiment Mustard Varieties for Production in Canada

By: Contributed by: Agriculture and Agri-Food Canada

Each year individuals, organizations, companies and institutions involved in the development, production, processing and marketing of varieties of grain crops in western Canada, come together for discussions at an Annual Meeting. This group discusses which new breeding lines will be recommended for registration and production in Canada and provides this recommendation to the Variety Registration Office (VRO) of the Plant Products Division, Canadian Food Inspection Agency (CFIA). This process has been going on for many years, with meetings organized on a rotational basis between the provinces of Manitoba, Saskatchewan and Alberta. Over the years, the face of Canadian agriculture has changed dramatically and this collective group has evolved its testing procedures and organizational structure in response to these and other changes.

The first official testing of condiment mustard varieties or breeding lines adapted for western Canada began in approximately 1963, using the procedures of the Expert Committee on Grains. Forty-three years later, in 2006, the testing, evaluation and exchange of information relevant to the development of improved cultivars of condiment mustard still continues using procedures approved by the members of the Prairie Recommending Committee for Oilseeds. The table below summarizes the data generated from seven years of testing varieties of the three types of condiment mustard produced in western Canada.

Condiment Table

Yield is measured on seed harvested from field plots (approximately 80 ft2 or 7.5 m2 area) with each entry replicated four times to improve the accuracy of the observation on the breeding lines and varieties. Although the seeding rate is similar to that of a producer, the yield is determined on the seed harvested from maintained research plots. The actual kg/ha determination does not always represent the equivalent yield achieved in a producer's field. Therefore, the yield relative to the standard or check cultivar is presented.

Seed size is identified as an issue of concern during the milling of condiment mustard and is also important to producers in the cleaning of seed and seedling establishment. New methods are being developed to assess the uniformity of seed size, but for the summary table, seed weight of 1000 seeds is reported.

Fixed oil or oil content of the seed is a criteria for assessment of a condiment mustard breeding line or variety. Sinapis alba, or yellow mustard, is a low oil content crop compared to the Brassica juncea species producing the brown or oriental types of mustard. The mustard industry prefers this relatively low oil content of both species of condiment mustard for processing. The composition of the oil is kept at current levels of erucic acid and the other long-chain fatty acids, to give the desired quality of the mustard product (R.K. Downey and G. Rakow, Harvest of Gold).

Protein content is included in the evaluation of mustard lines and the data reflects the inverse relationship between protein oil and oil content. Protein content of the seed becomes relevant for the purposes of processing when the seed is used as an ingredient in prepared meats.

The hot principle defines the condiment mustard product. When crushed seed comes in contact with moisture, the glucosinolate hydrolyses to produce the "heat" sensation in the mouth (J.S. Hemingway, The Mustard Species: Condiment and Food Ingredient Use and Potential as Oilseed Crops). While there is genetic variation for glucosinolate content, the hot, arid, climate of the Canadian prairies enables producers to grow mustard seed with high levels of glucosinolates. The hot principle for the yellow mustard, S. alba is derived from a different glucosinlate from that of the B. juncea sources of mustard types. Sinapis alba contains predominantly 4-hydroxybenzyl glucosinolate (sinalbin) while Brassica juncea types of condiment mustard contain predominantly 2-propenyl (allyl) glucosinolate (sinagrin) which hydrolyses to produce a volatile oil with strong pungency.

Mucilage content was recently included in the evaluation of mustard varieties as an important (processing and quality of product) characteristic for certain preparations of mustard. In addition to the emulsifying and suspension properties of mucilage, it is reported that mucilage may also give mustard its superior drought resistance (Balke and Diosday, Food Research International, 33(5) 347-56, 2000).

Seed colour is measured to assess degree of yellowness or brownness, of the seed coat of yellow or oriental mustard, and brown mustard respectively. Using reflectance-based methodology, a higher the (negative) value indicates brighter yellow of the seed coat while the brown seed colour imparts values closer to 0 or 1.

Distinct green seed and chlorophyll content are also assessed during the evaluation of condiment mustard. The same arid prairie climate that may grow beautiful coloured seed with high glucosinolate content, does not always give producers dry conditions at harvest time and early frosts may "fix" the cholorophyll content. This results in less-than-desirable quality of mustard seed for the processors (taste, color, oil and protein content) but it also causes economic losses for the producers through dockage. Genetic variation exists for these two traits and developers of germplasm select against elevated levels of green seed.

Current varieties of yellow mustard are not significantly different from each other for the traits of height and maturity, however, there is slight variation among the varieties of Brassica juncea.

When reading the data in the summary table, statistical comparisons are made within the species of condiment mustard. Thus, comparisons are made between S. alba or yellow mustard entries and comparisons are made between B. juncea, oriental and brown mustard entries. S.E.D. is the standard error of the difference between the average (mean) values for the traits evaluated and is used to calculate the F-value to determine the level of differences between the lines tested. The notation "ns" indicates that these differences are not statistically significant. Station-years is a term used to indicate the number of locations where the testing was done (one field test site in one year, at one location equals one station-year).

 

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